Abstract: Firstly, sodium alginate (SA) solutions with predetermined concentrations, and certain volume of 75% glycerol, 50% glutaraldehyde and model drug rhodamine B (4 g/L), were added into silk fibroin (SF) solution to form a series of homoge-neous mixtures which were used to prepare sustained-releasing silk fibroin/sodium alginate (SF/SA) membranes by casting method. Secondly, the preparation parameters were optimized via the response surface methodology aiming at achieving the highest product toughness (characterized as elongation at break). Lastly, the optimized SF/SA membrane’s toughness and the drug release kinetic model were tested and analyzed. We figured out the optimum preparation conditions for SF/SA membrane as: adding 0.96% (concentration) sodium alginate solution into an equal volume of 2.5% (concentration) silk fibroin solution, and setting the volume fractions of glycerol and glutaraldehyde to the whole mixture at 1.41% and 4.47%, respectively. And these optimized parameters could lead to a SF/SA membrane with an elongation at break of 228.36%, which was 18.14 times higher than that of the control group. The calculation result via regression equation suggested that the main factor affecting the elongation at break was glycerol dosage, and it had a significant synergistic effect with glutaraldehyde dosage. The drug release analysis confirmed the sustained-releasing cha-racter of the optimized group along with a pharmacokinetics coinciding with the Fickan model. The above results are favorable to the preparation of silk fibroin/sodium alginate membrane as the comfort and sustained-releasing dressing, which can provide reference for biomaterials application.
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2018, Vol. 32 
