基于丙烯酸-N-琥珀酰亚胺酯共聚物交联剂的壳聚糖水凝胶的生物相容性研究

doi:10.11896/cldb.22120119

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Abstract As a natural amino polysaccharide, chitosan has good biocompatibility and renewable resources, but its application is limited due to poor mechanical properties. In this study, acrylic acid, N-vinylpyrrolidone and acrylic acid N-succinimide ester were used as monomers to obtain terpolymer macromolecular crosslinker, which was mixed with chitosan solution to prepare chitosan hydrogel. The composition, morphology and thermal stability of the synthesized polymer and chitosan gel were characterized by infrared, thermogravimetric analysis and SEM. The results showed that the average molecular weight of acrylate-N-succinimide ester copolymer was 111 900, which was water soluble. The chitosan hydrogel with the copolymer crosslinker showed good mechanical properties, swelling properties and water vapor permeation rate. Compared with the gel membrane prepared under acidic conditions, the thermal decomposition temperature of the crosslinked gel membrane in PBS (pH=7.4) increased, the contact angle of water became larger, and the mechanical properties were greatly improved. Cytotoxicity experiments had shown that chitosan gels have good biocompatibility. Human adipose-derived stem cells formed cell clusters on the gels and secreted exosomes on the chitosan gel membrane. By covering the chitosan gel membrane over the stem cells, the cells grew normally and secreted exosomes. Chitosan gels therefore have the potential to be applied to skin dressings after loading exosomes.

Key words： chitosan acrylic acid-N-succinimide ester hydrogel exosome

Published: 25 June 2024 Online: 2024-07-17

CLC number:

TQ460.4

Fund:‘Six Talent Peaks' Team Project in Jiangsu Province (SWYY-CXTD-001), and International Cooperation Project Supported by Changzhou Science and Technology Bureau (CZ20190019).

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	Articles by authors
	YANG Shuiyan
	SHENG Yang
	SUN Yixin
	CAI Renqin
	Mark Bradley
	ZHANG Rong

Cite this article:

YANG Shuiyan,SHENG Yang,SUN Yixin, et al. Biocompatibility of Chitosan Hydrogels Based on Acrylic-N-Succinimidyl Ester Copolymer Crosslinker[J]. Materials Reports, 2024, 38(12): 22120119-10.

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http://www.mater-rep.com/EN/10.11896/cldb.22120119 OR http://www.mater-rep.com/EN/Y2024/V38/I12/22120119

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