| INORGANIC MATERIALS AND CERAMIC MATRIX COMPOSITES |
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| Study on the Inhibition of Tumor Cells by Deoxyelephantopin Loaded on Graphene Oxide Versus Nitrogen-doped Graphene Oxide Quantum Dots |
| HAN Xintong1, CAO Yang1,2, WEN Feng1, GAO Zhuwei3, LI Chengxin3, YU Xiaolong1,*
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1 School of Materials Science and Engineering, Hainan University, Haikou 570228, China
2 Key Laboratory of Child Cognition & Behavior Development of Hainan Province, Qiongtai Normal University, Haikou 571127, China
3 School of Chemical Engineering and Technology, Hainan University, Haikou 570228, China |
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Abstract Using bladder tumor cell UMUC-3 as a model, the anti-tumor activity of deoxyelephantopin (DEO) loaded on graphene oxide (GO) and nitrogen-doped graphene oxide quantum dots (NGQD) in vitro was studied. GO was prepared by Hummers method, NGQD was prepared by ball milling method, and DEO was connected onto GO and NGQD by ultrasonic loading. The grafting mechanisms of GO-DEO and NGQD-DEO were analyzed by FTIR. The microstructure and element distribution of GO-DEO and NGQD-DEO were analyzed by scanning electron microscope. MTT colorimetry was used to test the cell proliferation induced by GO-DEO and NGQD-DEO, and immunofluorescence was used to further analyze the reasons for NGQD-DEO inhibition. The results show that both GO and NGQD can load DEO by covalent bond grafting. In comparison, GO-DEO and NGQD-DEO groups can enhance the inhibition of tumor cells by destroying the cytoskeleton, with NGQD loading 15 μmol/L DEO for 48 h and 72 h were the best time to destroy the cytoskeleton.
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Published: 25 July 2023
Online: 2023-07-24
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| Fund:Hainan Provincial Platform Project Hainan Biomedical Material Research and Development Innovation Service Plat-form (Financing) Project(RZ2000007005). |
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2023, Vol. 37 
