基于石墨烯/氯化血红素复合物纳米酶可视化检测谷胱甘肽

doi:10.11896/cldb.22030004

Abstract
Figure/Table
References
Related Citation (15)

Download:

Full Text ( PDF ) (5336KB)
Export: BibTeX | EndNote (RIS)

Abstract Nano-enzyme is a kind of nano-materials with enzyme-like catalytic activity, which is similar to natural enzymes in size, shape and surface charge. Nano-enzyme is expected to be a substitute for natural enzyme because of its simple preparation, low cost and good stability. Nano-enzyme has a broad application prospect in biological analysis, imaging, treatment, environmental protection and so on. Graphene has a broad application prospect as a two-dimensional catalyst carrier because of its large specific surface area, high electrical and thermal conductivity, good mechanical properties, few lattice defects and rich surface functional groups. In this work, a kind of nanocomposite GR/Hemin with high enzyme activity was prepared by self-assembly of graphene and hemin. The composite retains the characteristics of large specific surface area of graphene and can catalyze oxygen molecules to form free radicals (ROS) under acidic conditions, thus oxidizing TMB. Based on the competitive relationship between glutathione (GSH) and TMB oxidation in the system, a rapid and sensitive visual method for GSH detection was established. This method shows a good linear relationship between UV absorbance at 652 nm and glutathione concentration in the range of 0.2—100 μmol/L, with the detection limit of 0.059 μmol/L. The lowest glutathione concentration recognizable to the naked eye is 20 μmol/L. It can realize the simple, rapid and highly selective detection of GSH in serum, and has a broad application prospect in clinical diagnosis.

Key words： glutathione graphene tetramethylbenzidine (TMB) visual detection

Published: Online: 2023-02-08

CLC number:

TB332

Fund:National Natural Science Foundation of China (21972102,21904092),and National Key R & D Program of China (2021YFA0910400).

Corresponding Authors: guyu@usts.edu.cn;ecmli@swu.edu.cn;cxguo@usts.edu.cn

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	FAN Cunxia
	GU Yu
	QIU Xingchen
	LI Changming
	GUO Chunxian

Cite this article:

FAN Cunxia,GU Yu,QIU Xingchen, et al. Visual Detection of Glutathione Based on Graphene/Hemin Composites with Nanoenzymes Activity[J]. Materials Reports, 2023, 37(2): 22030004-8.

URL:

http://www.mater-rep.com/EN/10.11896/cldb.22030004 OR http://www.mater-rep.com/EN/Y2023/V37/I2/22030004

1 Garcia-Viloca M, Gao J, Karplus M, et al. Science, 2004, 303 (5655), 186.
2 Manea F, Houillon F B, Pasquato L, et al. Angewandte Chemie International Edtion in English, 2004, 43 (45), 6165.
3 Zeng R, Luo Z, Zhang L, et al. Analytical Chemistry, 2018, 90 (20), 12299.
4 Li Y S, Chen L. Journal of Inorganic Materials, 2018, 33 (2), 221.
5 Wang J J, Wang J L, Zhou P, et al. Mikrochim Acta, 2020, 187 (2), 99.
6 Yin Z, Shi Y, Cen M, et al. Science Bulletin, 2021, 66 (15), 1518.
7 Li N, Huang M, Zhou J, et al. Chinese Journal of Catalysis, 2021, 42 (5), 781.
8 Wu W, Wang Q, Chen J, et al. Nanoscale, 2019, 11 (26), 12603.
9 Chen Y Y, Zhao C X, Wang Y Y, et al. Materials Science & Engineering C-Materials For Biological Applications, 2020, 117, 111264.
10 Dugan L L, Gabrielsen J K, Shan P Y, et al. Neurobiology of Disease, 1996, 3 (2), 129.
11 Cao G, Sun D, Gu T, et al. Biosensors and Bioelectronics, 2019, 145, 111707.
12 Kong Z K, Chen Y, Hua J Z, et al. New Carbon Materials, 2021, 36 (4), 810.
13 Wang B, Ding Y, Deng Z, et al. Chinese Journal of Catalysis, 2019, 40 (3), 335.
14 Song Y, Qu K, Zhao C, et al. Advanced Materials, 2010, 22 (19), 2206.
15 Wang Q, Lei J, Deng S, et al. Chemical Communications, 2013, 49 (9), 916.
16 Ji J, Li Y, Peng W, et al. Advanced Materials, 2015, 27 (36), 5264.
17 Jiao L, Wu J, Zhong H, et al. ACS Catalysis, 2020, 10 (11), 6422.
18 Li R, Zhen M, Guan M, et al. Biosensors and Bioelectronics, 2013, 47, 502.
19 Wu W, Wang Q, Chen J, et al. Nanoscale, 2019, 11 (26), 12603.
20 Xie J W, Cheng D, Li P P, et al. ACS Applied Nano Materials, 2021, 4 (5), 4853.
21 Petrini S, D'Oria V, Piemonte F. Methods in Molecular Biology, 2015, 1208, 395.
22 Tcherkas Y, Denisenko A. Journal of Chromatography A, 2001, 913 (1-2), 309.
23 Burford N, Eelman M D, Mahony D E, et al. Chemical Communications, 2003, 7(1), 146.
24 Li S, Zhao X T, Gang R T, et al. Analytical Chemistry, 2020, 92 (7), 5152.
25 Chen Y F, Jiao L, Yan H Y, et al. Analytical Chemistry, 2020, 92 (19), 13518.
26 Olabi A G, Abdelkareem M A, Wilberforce T, et al. Renewable and Sustainable Energy Reviews, 2021, 135, 110026.
27 Shaari N, Kamarudin S K. Renewable and Sustainable Energy Reviews, 2017, 69, 862.
28 Xu J, Huang J, Wang Z, et al. Chinese Journal of Catalysis, 2020, 41 (3), 474.
29 Liu Y, Ding J, Wang Q, et al. New Carbon Materials, 2021, 36 (4), 779.
30 Banerjee A N. Interface Focus, 2018, 8 (3), 20170056.
31 Madni A, Noreen S, Maqbool I, et al. Journal of Drug Targeting, 2018, 26 (10), 858.
32 Cai L, Hu C, Liu S, et al. Science China Materials, 2021, 64 (2), 488.
33 Zhang H, Chen J, Yang Y, et al. Analytical Chemistry, 2019, 91 (8), 5004.
34 Wang N, Xie M, Wang M, et al. Talanta, 2020, 220, 121352.
35 Wang J, Wang X, Pan X, et al. Microchimica Acta, 2020, 187 (6), 1.
36 Meyer J C, Geim A K, Katsnelson M I, et al. Nature, 2007, 446 (7131), 60.
37 Qi H, Cao L Y, Li J Y, et al. ACS Applied Materials & Interfaces, 2016, 8 (51), 35253.
38 Gu Y, Fan C, Hao X, et al. Advanced Functional Materials, 2022, 32 (9), 2110192.
39 Guo Y, Li J, Dong S. Sensors and Actuators B: Chemical, 2011, 160 (1), 295.
40 Hui L, Xue Y, Yu H, et al. Chemphyschem, 2020, 21 (19), 2145.
41 Gang W, Gu Z Y, Gong S S, et al. Spectroscopy and Spectral Analysis, 2020, 40 (6), 1722.
42 Sohrabi S, Dehghanpour S, Ghalkhani M. ChemCatChem, 2016, 8 (14), 2356.
43 Zhang L, Hou Y, Guo X Y, et al. Luminescence, 2020, 35 (8), 1350.
44 Jiang N, Zhang C, Li M, et al. Micromachines (Basel), 2021, 12 (9), 1099.