羧甲基壳聚糖-羧基葡聚糖凝聚微滴的制备及其酵母细胞毒性

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Abstract Coacervate microdroplets can form an artificial cell wall on the cell surface via self-assembly, which can protect the cell and decrease the damage caused by external stimulation and chemical environment. Natural polymers with advantages such as good biocompatibility and biodegradability, can be employed to prepare coacervate microdroplets with stable structure. It is of great significance to explore the preparation methods of coacervate microdroplets and their application in the cell protection. In this paper, chitosan and dextran were used to prepare the basic element materials of coacervate microdroplets, carboxymethyl chitosan (CMCS) and carboxyl dextran (DEX-COOH), which were employed to construct the coacervate microdroplets. Some techniques such as Fourier Transform Infrared Spectrometer (FTIR), UV-visable spectrophotometry (UV-vis), fluorescent inverted microscope (FIM), confocal laser scanning microscope (CLSM) and dynamic light scattering (DLS) were used to characterize the structure of coacervate microdroplets. The results showed that the stability of the coacervate microdroplets prepard under the condition that the mass concentration ratio of CMCS and DEX-COOH was 1∶2. Without cytotoxicity to saccharomyces cerevisiae, the prepared coacervate microdroplets present potential applications in the improvement of probiotics resistance to external stimulation.

Key words： carboxymethyl chitosan carboxyl dextran coacervate microdroplets probiotics cytotoxicity

Published: 08 January 2021

CLC number:	O636.1
	Q735

Fund:This work was financially supported by the National Natural Science Foundation of China (51663020).

About author:: Hanjiao Hu studied at Guangxi University with a master's degree in September 2017. From September 2018 to January 2020, she co-educated and learned at Harbin Institute of Technology, the research direction is the protection and enhancement of microbial probiotics. Yanzhen Yin, professor at Beibu Gulf University, part-time doctoral/master tutor of Guangxi University. He graduated from the School of Chemistry of Jilin University in July 2011 with a Ph.D. degree in polymer chemistry and physics. Since joining Beibu Gulf University in the same year, he has been mainly engaged in the research on the green processing of marine waste resources and the functional utilization of selenium-rich, coastal chemical pollutants monitoring and prevention. In the past five years, he published 6 SCI papers and applied for 1 invention patent.

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	HU Hanjiao
	LIANG Xingtang
	WANG Shuangshuang
	ZHANG Xia
	YIN Yanzhen
	YIN Xuebin

Cite this article:

HU Hanjiao,LIANG Xingtang,WANG Shuangshuang, et al. Preparation of Coacervate Microdroplets Based on Carboxymethyl Chitosan and Carboxyl Dextran and Its Cytotoxicity Towards Yeast[J]. Materials Reports, 2020, 34(Z2): 496-500.

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http://www.mater-rep.com/EN/ OR http://www.mater-rep.com/EN/Y2020/V34/IZ2/496

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