盐屋链霉菌发酵条件优化及抗病机理研究

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Abstract To reveal the mechanisms of resistance to cytospora chrysosperma of biocontrol strain F57 (Streptomyces sioyaensis), the effects of fermentation broth of Streptomyces on the induced resistance was studied, and the optimal fermentation medium and fermentation conditions of strain F57 were defined meanwhile, in this study. The optimum fermentation conditions in the Shaker with different nutrient sources were selected through designing orthogonal experiment by using the Valsasordidaas indicators. The annual Populuspyramidalis cv. Huangkuai cuttings were inoculated with Valsasordida by the pinprick method, and the activity of the PAL, the changes of the contents of flavonoids, total phenol, proline and lignin in the leaves were determined by colorimetry. The results showed that the best carbon source is the soluble starch, the best organic nitrogen source is peanut powder and ammonium sulfate is the best inorganic nitrogen source. The optimum medium formula for biocontrol Streptomyces strain F57 was expressed as the following: 70 g soluble starch, 25 g peanut powder, 2 g yester powder, 3 g NH₄NO₄, 2.7 g CaCO₃, 2.7 g NaCl, 1 000 mL water, at pH 7.2. The optimum conditions for fermentation in shake-flask were defined as follows: the fermentation temperature was 28 ℃; initial pH at 7.0; the fermentation time was 5 days; five-day old strains cultivated on medium for seed broth preparation; the volume of broth in a 250 mL flask was 40 mL and inoculation amount was 3%. The results of disease resistance mechanism showed that: after treated with fermentation broth, the contents of proline and lignin were increased in poplar and played a role in protective and therapeutic on the pathogen of poplar canker, which indicated that the fermentation broth has a therapeutic effect on the pathogen of poplar canker. The results on the metabolic pathway of phenylpropanoid metabolism in plants showed that spraying fermentation broth could increase the activity of PAL and cause the contents of flavonoid and total phenol increase, thus enhancing the disease resistance in poplar. In conclusion the fermentation broth of Streptomyces could inhibit pathogenic bacteria. Spraying the fermentation after bacteria invasion could increase the activity of PAL and the contents of proline, lignin, flavonoid and total phenol, thus enhancing the resistance in poplar.

Key words： cytospora chrysosperma induced resistance streptomyces sioyaensis fermentation

Published: 25 November 2019

CLC number:

S476

Fund:This work was financially supported by Demonstration Project of Forestry Science and Technology Promotion of Central Treasury (JLT-2016-6), Special Bids of Major Technical Projects of Department of Science and Technology of Jilin Province (20150203015NY) and the Project of Forestry Science and Technology of Forestry Department of Jilin Province (2015-004).

About author:: † These authors contributed equally to this work.
Tiantian Zheng obtained her master’s degree in microbiology from Liaoning University in 2017.She is currently working in Tobacco Research Institute of Chinese Academy of Agricultural Sciences. Her job focus on the mechanism of resistance to tobacco brown spot. She has published 6academic papers, authorized 1 national invention patent.
Yan Sui received her bachelor of science in biotechno-logy from the Liaoning University in 2017.She is studying for a master’s degree at Wuhan Institute of Virology, Chinese Academy of Sciences. She has published 1 academic paper and authorized 1 national invention patent.
Limei Li obtained her Ph.D. degree in Plant pathology from the Shenyang Agricultural University in 2010.She is currently a master instructor and an associate research of the Jilin Provincial Academy of Forestry Sciences.Her research direction focus on the forest pathology and biological control. As the backbone of the project, she has taken charge of and participated in more than 10 scientific research projects, and two of which were rewarded with the Third of the Scientific and Technological Progress Prize. She has published more than 20 journal papers and authorized 2 national invention patents and more than 10 national utility model patents.

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	ZHENG Tiantian
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	ZHU Chunyu
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Cite this article:

ZHENG Tiantian,SUI Yan,CHEN Yuequ, et al. Optimization of Fermentation Conditions and Study on Resistance Mechanism ofStreptomyces Sioyaensis[J]. Materials Reports, 2019, 33(Z2): 559-567.

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http://www.mater-rep.com/EN/ OR http://www.mater-rep.com/EN/Y2019/V33/IZ2/559

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