多孔胶原-β-磷酸三钙-硫酸软骨素复合膜的制备与表征<sup>*</sup>

doi:10.11896/j.issn.1005-023X.2017.02.002

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Abstract The porous collagen-β-tricalcium phosphate-chondroitin sulfate composite membrane was prepared by EDC modification and twice freeze-dried method. The effect of variation of material proportion, pH value and method in materials preparation process on the structure and properties of composite materials were investigated by scanning electron microscope (SEM), X-ray diffraction analyzer (XRD) and atomic force microscope (AFM), FT-IR, porosity measurer, MTT cytotoxicity tester. The results indicated that when the pH value of collagen/HCl solution was 2, the mass ratio of collagen and β-tricalcium phosphate was 1∶2 (m(Col)∶m(β-TCP)=1∶2), the β-TCP′s crystalline phase maintained, the composition was uniform and compact. After EDC modification, the SEM and AFM results showed that the collagen bundles after crosslinking got bigger and thicker obviously, arranged closely in a certain direction. XRD spectrum showed that the characteristic diffraction peaks of β-TCP in composite materials were obvious. The pore size of composite material was about 80-90 μm, the porosity of ternary membrane was (90.76±1.28)%, larger than that of collagen membrane (85.88±0.91)%. Infrared spectra confirmed that Ca²⁺ of β-TCP and carboxyl of Col were chemically bonded. AFM showed that β-TCP particles had a direct connection with collagen. The MTT cytotoxicity levels of compo-site materials were 1, which was a kind of potential dental restorative material.

Key words： collagen β-tricalcium phosphate chondroitin sulfate porous composite membrane microstructure

Published: 02 May 2018

CLC number:

O636

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	Articles by authors
	XUE Yuan
	DAN Nianhua
	DAN Weihua

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XUE Yuan,DAN Nianhua,DAN Weihua. Preparation and Characterization of Porous Collagen-β-tricalcium Phosphate-chondroitin Sulfate Composite Membrane[J]. Materials Reports, 2017, 31(2): 8-12.

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http://www.mater-rep.com/EN/10.11896/j.issn.1005-023X.2017.02.002 OR http://www.mater-rep.com/EN/Y2017/V31/I2/8

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