Materials Reports  2020, Vol. 34 Issue (Z1): 97-102 |
| INORGANIC MATERIALS AND CERAMIC MATRIX COMPOSITES |
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| Preparation and Properties of Activable Fluorescence-Magnetic ResonanceDual-mode Nanoprobe |
| GUO Xiao1, ZHOU Yujie1, GAO Jingru1, YU Wei1, XU Cui1, HAN Cuiping1,2
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1 School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China;
2 Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China |
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Abstract In this work, fluorescent carbon dots with the quantum yield of 93% were prepared using a solvent-free method by carbonization of sodium citrate and urea at 200 ℃ for 1 h. The prepared CDs and MnO2 nanosheets were electrostatically assembled to construct activated fluorescence-magnetic resonance dual-modal MnO2-CDs using poly (allylamine hydrochloride) (PAH) as coupling agent. The preparation of MnO2-CDs was optimized to improve the imaging performance. The activation of fluorescence and magnetic resonance signals of MnO2-CDs was investigated. In the presence of GSH, the fluorescence was restored, and the T1-weighted signal was enhanced with a relaxation rate of 7.01 mmol-1·L·s-1. When the concentration of the material was as high as 2 mg/mL, the survival rate of the cells was still above 85%. The HO-8910 ovarian cancer cells showed bright fluorescence and T1-weighted signals after incubation with MnO2-CDs, while Ea.hy926 normal cells showed only weak signals.The fluorescence and magnetic resonance bimodal GSH-activatable nanoprobe MnO2-CDs not only had high quantum yield and T1 relaxation efficiency, but also had low cytotoxicity. Moreover, these nanoparticles could allow for the detection of cancer cells with both fluorescence and MRI imaging techniques through the GSH activated signals would greatly increase the target-to-background signal ratio for higher imaging sensitivity.
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Published: 01 July 2020
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| Fund:This work was financially supported by the National Natural Science Foundation of China (81671744),China Post-doctoral Fund Surface Project (2016M591923, 2017T100405),the Project of Invigorating Health Care through Science, Technology and Education, Jiangsu Provincial Medical Youth Talent (QNRC2016782),the Peak of Six Talents of Jiangsu Province (WSW-051),Innovation and Entrepreneurship Training Program for College Students in Jiangsu Province (201710313004Z). |
| About author:: Xiao Guo is studying for master's degree at Xuzhou Medical University from September 2014 to June 2019, he obtained a bachelor's degree in medical imaging from Xuzhou Medical University. He was an outstanding graduate of the 2019 Xuzhou Medical University. After graduation, he continued to study imaging medicine and nuclear medicine at Xuzhou Medical University. One core Chinese Journal was published by the first author, one SCI journal by the third author, and one national journal by the third author. Served as the first person in charge of the provincial key project of 2017 college students' innovation and entrepreneurship training program “Research on fluorescence MRI molecular imaging of ovarian cancer cells with double targets and can activate probes”, and successfully concluded the topic ; Cuiping Han, doctor, master's supervisor, the third level talent of “333 project” in Jiangsu Province, the high level talent of “six talent peaks” in Jiangsu Pro-vince (Class C), the outstanding young backbone teacher of “Blue Project” in Jiangsu Universities, the young talent of “science and education strengthening health project” in Jiangsu Province, and the young member of digital medicine branch of Jiangsu Medical Association. She is mainly engaged in the research of nano biomedical technology and molecular image, and presided over 9 scientific research projects, including general program of National Natural Science Foundation, youth program of National Natural Science Foundation, Jiangsu Natural Science Foundation, China Postdoctoral fund and Jiangsu postdoctoral fund. 19 SCI research papers were published as the first author or correspon-ding author. To guide 2 key projects and 2 general projects of national innovation training plan for college students. Assist and guide 10 postgraduate students. |
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