| POLYMERS AND POLYMER MATRIX COMPOSITES |
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| Biocompatibility Assessment and Chondrogenic Differentiation of Poly-L-lactide-based Nanofibrous Scaffolds |
| HONG Yazhen1,2, YANG Dingzhu1,2
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1 College of Chemical Engineering, Huaqiao University, Xiamen 361021;
2 Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021 |
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Abstract Initially, we prepared poly-L-lactide (PLLA)-based porous scaffolds, and then the biosafety, as well as chondrogenic differentiation potential of the same, were evaluated. The biocompatibility of nanofibrous scaffolds was assessed by using various tests in different models. At first, the cytotoxicity was measured using L929 as cell model using Alamar Blue assay. In addition, the acute systemic toxicity was measured by injecting scaffold in the form of extracts into KM mice. Furthermore, hemolysis test of the scaffold was investigated using fresh blood drawn from rabbit as blood model. The scaffolds resulted in low cytotoxicity, no acute toxicity in mice, and highly compatible with the blood. Eventually, the chondrogenic differentiation potential of C5.18 cells on the designed scaffolds with diversified topology was determined. In addition, dexamethasone (DEX) was used for the enhancement of differentiation process as it favors the differentiation of C5.18 cells at 10-6 mol/L of DEX. The degree of cartilage differentiation and the production of glycosaminoglycans and collagen protein was augmented with the increase in induction time. These nanofibrous scaffolds can serve as a prospect material for cartilage tissue construction with a unique advantage in promoting the differentiation of chondrogenesis.
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Published: 18 October 2018
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1 Deng A H, Chen A Z, Wang S B, et al. Porous nanostructured poly-L-lactide scaffolds prepared by phase inversion using supercritical CO2 as a nonsolvent in the presence of ammonium bicarbonate particles[J]. The Journal of Supercritical Fluids,2013,77:110.
2 Yang D Z, Chen A Z, Wang S B, et al. Preparation of poly(L-lactic acid) nanofiber scaffolds with a rough surface by phase inversion using supercritical carbon dioxide[J]. Biomedical Materials,2015,10(3):035015.
3 Oyama H T, Tanishima D, Maekawa S. Poly(malic acid-co-L-lactide) as a superb degradation accelerator for poly(L-lactic acid) at physiological conditions[J]. Polymer Degradation and Stability,2016,134:265.
4 Lizundia E, Mateos P, Vilas J L. Tuneable hydrolytic degradation of poly(L-lactide) scaffolds triggered by ZnO nanoparticles[J]. Mate-rials Science and Engineering C,2017,75:714.
5 Huang Y W, Fan X Z. Chitosan/polylactic acid complex for repair of peripheral nerve defects[J]. Chinese Journal of Tissue Engineering Research,2015,19(25):4059(in Chinese).
黄永旺,范学政.几丁糖与聚乳酸合成生物材料修复周围神经缺损[J].中国组织工程研究,2015,19(25):4059.
6 Liu Y G, Zhou X T. Polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold repairs laryngeal cartilage defect[J]. Chinese Journal of Tissue Engineering Research,2015,19(52):8379(in Chinese).
刘永刚,周香桃.聚乳酸-乙醇酸共聚物/羟基磷灰石复合支架修复喉软骨缺损[J].中国组织工程研究,2015,19(52):8379.
7 Badaraev A D, Nemoykina A L, Bolbasov E N, et al. PLLA scaffold modification using magnetron sputtering of the copper target to provide antibacterial properties[J]. Resource-Efficient Technologies,2017,3(2):204.
8 Huang K C, Yano F, Murahashi Y, et al. Sandwich-type PLLA-nanosheets loaded with BMP-2 induce bone regeneration in critical-sized mouse calvarial defects[J]. Acta Biomaterialia,2017,59:12.
9 Santos D, Silva D M, Gomes P S, et al. Multifunctional PLLA-ceramic fiber membranes for bone regeneration applications[J]. Journal of Colloid and Interface Science,2017,504:101.
10 Wang Q, Shao W L, Gao Y F, et al. Biomimetic mineralization and biocompatibility of PLGA/TSF electrospun nanofibers[J]. Journal of Materials Science and Engineering,2017,35(2):253(in Chinese).
王倩,邵伟力,高艳菲,等.PLGA/TSF静电纺纳米纤维的仿生矿化及生物相容性[J].材料科学与工程学报,2017,35(2):253.
11 Bao G J, Zhong N, Zhang W X, et al. Research on the performance of PGS/PLLA electrostatic spinning of composite nanofiber scaffold[J]. Materials Review B: Research Papers,2016,30(7):67(in Chinese).
包广洁,钟妮,张文霞,等.PGS/PLLA静电纺丝复合纳米纤维支架的性能研究[J].材料导报:研究篇,2016,30(7):67.
12 Martinez E, Engel E, Planell J A, et al. Effects of artificial micro- and nano-structured surfaces on cell behaviour[J]. Annals of Anatomy-Anatomischer Anzeiger,2009,191(1):126.
13 Palin E, Liu H N, Webster T J. Mimicking the nanofeatures of bone increases bone-forming cell adhesion and proliferation[J]. Nanotechnology,2005,16(9):1828.
14 Dalby M J, McCloy D, Robertson M, et al. Osteoprogenitor response to defined topographies with nanoscale depths[J]. Biomate-rials,2006,27(8):1306.
15 Chen W M. Three-dimensional porous scaffolds based on gelatin/polylactic acid nanofibers for articular cartilage tissue regeneration[D]. Shanghai: Donghua University,2017(in Chinese).
陈维明.明胶/聚乳酸纳米纤维三维多孔支架的制备及应用于关节软骨组织再生[D].上海:东华大学,2017.16 Cao J L. Relationship of proteoglycan with the structure and function of cartilage and osteoarthropathy[J]. Journal of Xi’an Jiaotong University(Medical Sciences),2012,33(2):131(in Chinese).
曹峻岭.蛋白聚糖与软骨结构、功能及骨关节病的关系[J].西安交通大学学报(医学版),2012,33(2):131.
17 Gong Y H. Preparation and modification of poly L-lactic acid porous scaffold and manufacture of tissue engineering cartilage[D]. Hangzhou: Zhejiang University,2006(in Chinese).
龚逸鸿.聚乳酸多孔支架的制备、改性及组织工程化软骨的构建[D].杭州:浙江大学,2006.
18 Grigoriadis A E, Heersche J N M, Aubin J E. Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexamethasone[J]. The Journal of Cell Biology,1988,106:2139.
19 Deng A H. Fabrication of the SF/PLLA compound tissue enginee-ring scaffolds by supercritical fluids technology[D]. Xiamen: Huaqiao University,2013(in Chinese).
邓爱华.超临界流体技术制备丝素/聚乳酸复合组织工程支架的研究[D].厦门:华侨大学,2013.
20 ISO.10993-2009. Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity[S]. Switzerland: ISO copyright office,2007.
21 全国医疗器械生物学评价标准化技术委员会.GB/T 16886.11-2011医疗器械生物学评价第11部分:全身毒性试验[S].北京:中国标准出版社,2011.
22 全国医疗器械生物学评价标准化技术委员会.GB/T 16886.12-2005医疗器械生物学评价第12部分:样品制备与参照样品[S].北京:中国标准出版社,2005.
23 Mark K V D, Gauss V, Mark H V D, et al. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture[J]. Nature,1977,267(5611):531.
24 Chang W, Tu C, Komuves L, et al. Calcium sensing in cultured chondrogenic RCJ3.1C5.18 cells[J]. Endocrinology,1999,140(4):1911.
25 Talukdar S, Nguyen Q T, Chen A C, et al. Effect of initial cell seeding density on 3D-engineered silkfibroin scaffolds for articular cartilage tissue engineering[J]. Biomaterials,2011,32:8927. |
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2018, Vol. 32 
