D-氨基酸驱散生物膜的行为与作用机理研究

doi:10.11896/cldb.21050076

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Abstract In order to clarify the biofilm dispersing effect and bactericidal mechanism of D-amino acid enhanced biocide, different D-amino acids were used in this work to form a compound biocides with traditional biocides tributyl tetradecyl phosphonium chloride(TTPC), tetramethyl-phosphate sulfate(THPS) and antimicrobial peptides. By means of weightloss experiment, electrochemical test and surface analysis, the bactericidal inhibition effect of compound biocide on sulfate reducing bacteria(SRB), iron oxidizing bacteria(IOB) and the mixture of SRB+IOB on the surface of carbon steel was studied, and the behavior and mechanism of dispersive biofilm by D-amino acid were determined. The results show that SRB and IOB cooperate to form dense biofilms on the surface of carbon steel samples, which not only provides an anaerobic environment suitable for the growth of SRB, but also plays a certain protective role on SRB, which will lead to the most serious corrosion caused by the mixed bacteria of SRB+IOB. The biofilm dispersal signal factor released by D-amino acid can change the peptidoglycan composition of bacterial cell wall and regulate the expression of cell genes, inhibit the formation of biofilm by binding with bacterial proteins, and make the existing biofilm actively disperse off the surface of carbon steel, and destroy the oxygen concentration difference environment formed by SRB and IOB. D-amino acid to a large extent inhibits the aggravation of metal corrosion caused by extracellular polymeric substances(EPS) produced by bacteria, and thus enables biocides to better kill bacteria under the biofilm. The biocidal rates of SRB and IOB in the mixed biofilm are up to 100% and 82.60%, respectively. The results showed that D-amino acid had a good bactericidal enhancement effect on the biocide by dispersing the biofilm behavior.

Key words： D-amino acid mixed bacteria biofilm sulfate reducing bacteria(SRB) iron oxidizing bacteria(IOB) carbon steel bactericidal enhancement effect

Published: 10 January 2023 Online: 2023-01-31

CLC number:

TG172.4

Fund:Key Research and Development Program of Shaanxi Province(2020GY-234), the National Natural Science Foundation of China(51974245, 21808182), Xi’an Key Laboratory of High Performance Oil and Gas Field Materials, School of Material Science and Engineering, Xi’an Shiyou University, Xi’an Shiyou University Materials Science and Engineering Provincial Advantageous Disciplines(YS37020203), Xi’an Shiyou University Gra-duate Student Innovation and Practice Development Program(YCS20112020,YCS20112021).

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	Articles by authors
	XU Congmin
	GAO Haoran
	ZHU Wensheng
	YANG Xing
	CHEN Yueqing
	WANG Wenyuan

Cite this article:

XU Congmin,GAO Haoran,ZHU Wensheng, et al. Study on the Behavior and Mechanism of D-amino Acid Dispersing Biofilm[J]. Materials Reports, 2023, 37(1): 21050076-7.

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http://www.mater-rep.com/EN/10.11896/cldb.21050076 OR http://www.mater-rep.com/EN/Y2023/V37/I1/21050076

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