Abstract: The novel temperature-sensitive porous hydrogel was synthesized by using free radical polymerization method, the raw materials of hydroxyethyl methacrylate (HEMA) and N, N-diethyl-acrylamide (DEAAm) were use as reactants. Then propyl was introduced by grafting reaction. The intermediate product and the final product were characterized by infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (NMR), scanning electron microscope (SEM). The swelling and desorption kinetics of the final product were studied by weighing method, and the swelling properties and temperature response were also analyzed. Following the principle of ‘click reaction’, the creatine kinase was fixed on the matrix material of novel temperature-sensitive semi-interpenetrating porous hydrogel. The enzyme loading was measured by BCA (bicinchoninic acid) protein kit method, and the enzyme activity was measured by pH method. The experimental results showed that the novel temperature-sensitive hydrogels was successfully synthesized and the average loading of creatine kinase was 24.77 mg·g-1, the average activity of creatine kinase was 9.67 U·g-1. The novel temperature-sensitive hydrogel has good selectivity and temperature response to creatine kinase, which is of great reference for the effective utilization of creatine kinase.
黄婧欣, 曾楚楚, 郭明. 新型温敏网络半互穿多孔水凝胶的制备及其固定化酶的研究*[J]. 《材料导报》期刊社, 2017, 31(21): 158-163.
HUANG Jingxin, ZENG Chuchu, GUO Ming. Preparation of the Novel Temperature-sensitive Semi-interpenetrating Porous Hydrogel and the Use in Terms of Immobilized Enzyme. Materials Reports, 2017, 31(21): 158-163.
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