多孔CoNi/NC纳米酶的制备及在比色法检测Mn<sup>2+</sup>中的应用研究

doi:10.11896/cldb.25020005

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Abstract Porous CoNi bimetallic nitrogen-doped carbon nanozyme (CoNi/NC) with oxidase-like activity was prepared by high-temperature calcination. This nanozyme can directly catalyze the chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine(TMB) to produce a blue coloration wit-hout the need for H₂O₂. The excellent peroxidase-like catalytic performance of this nanozyme is attributed to the synergistic effect of its bimetallic components and its porous structure. Kinetic studies revealed that the K_m value is (0.919±0.283) mmol/L, and the V_max is (3.850±0.707)×10^-8 mol·L^-1·s^-1, indicating strong substrate affinity and a high reaction rate. Free radical scavenging experiments demonstrated that ·OH and ·O₂^- radicals play crucial roles in the oxidation and color change process of TMB. Leveraging the phenomenon that Mn²⁺ can complex with the chromogenic product to cause solution decolorization, we developed a novel colorimetric method for detecting Mn²⁺ content. This method exhibits a linear range of 0.005—0.04 mmol/L and a detection limit as low as 0.001 7 mmol/L, and has been successfully applied to real water samples. To enhance the practicality of this method, the nanozyme was immobilized in a gel matrix, exploring its potential for detecting actual samples and providing new insights into the integration of colorimetric detection methods.

Key words： porous CoNi/NC nanozyme oxidase-like activity colorimetric method Mn²⁺ detection

Published: Online: 2026-02-13

CLC number:	X832
	O657.3

Corresponding Authors: zjuwxd@mail.usts.edu.cn;liutt@mail.usts.edu.cn

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	Articles by authors
	GONG Hailong
	SHEN Siyu
	HOU Xiaofeng
	MA Qinqin
	WANG Xuedong
	LIU Tingting

Cite this article:

GONG Hailong,SHEN Siyu,HOU Xiaofeng, et al. Preparation of Porous CoNi/NC Nanozyme and Its Application in Colorimetric Detection of Mn²⁺[J]. Materials Reports, 2026, 40(3): 25020005-8.

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https://www.mater-rep.com/EN/10.11896/cldb.25020005 OR https://www.mater-rep.com/EN/Y2026/V40/I3/25020005

1 Michalke B, Fernsebner K. Journal of Trace Elements in Medicine and Biology, 2014, 28(2), 106.
2 Rezvani S A, Soleymanpour A. Journal of Chromatography A, 2016, 1436, 34.
3 Dao Z L, Zhao Y, Yi L H, et al. Physical Testing and Chemical Analysis Part B:Chemical Analysis, 2022, 58(3), 361(in Chinese).
刀朱俐, 赵瑜, 尹利辉, 等. 理化检验-化学分册, 2022, 58(3), 361.
4 Zhang J M, Chen X X, Li Y, et al. Analytical Methods, 2018, 10(5), 541.
5 Chen L L, Zhang P Y. Chinese Journal of Environmental Engineering, 2013, 7(9), 6(in Chinese).
陈立雷, 张培玉. 环境工程学报, 2013, 7(9), 6.
6 Heo J, Hwang C. Nanomaterials, 2016, 6(5), 82.
7 Yang D D, Zhang X L, Cui C E, et al. Journal of Instrumental Analysis, 2015, 34(10), 1179(in Chinese).
杨冬冬, 张校亮, 崔彩娥, 等. 分析测试学报, 2015, 34(10), 1179.
8 Gao L, Zhuang J, Nie L, et al. Nature Nanotechnology, 2007, 2(9), 577.
9 Chen Y, Lai Z C, Zhang X, et al. Nature Reviews Chemistry, 2020, 4(5), 243.
10 Zur K K, Farajpour A. Nanomaterials, 2022, 12(3), 476.
11 Xi J Q, Wei G, An L F, et al. Nano Letters, 2019, 19(11), 7645.
12 Li X, Ding S, Lyu Z, et al. Small, 2022, 18(37), 22030.
13 Chen Y, Jiang B, Hao H, et al. Angewandte Chemie International Edition, 2023, 62(19), e20230187.
14 Huang L, Chen J, Gan L, et al. Science Advances, 2019, 5(5), eaav5490.
15 Losada-Garcia N, Jimenez-Alesanco A, Velazquez-Campoy A, et al. ACS Applied Materials & Interfaces, 2021, 13(4), 5111.
16 Chen J, Ma Q, Li M, et al. Small, 2023, 19(8), 2205924.
17 Cheng C, Wang H, Zhao J, et al. Colloids and Surfaces B:Biointerfaces, 2024, 235, 113767.
18 Sun Y, Xu B, Pan X, et al. Coordination Chemistry Reviews, 2023, 475, 214896.
19 Pi Z J, Zhang Y Y, Chen L, et al. Materials Reports, 2024, 38(S2), 24100085(in Chinese).
皮子杰, 张园园, 陈龙, 等. 材料导报, 2024, 38(S2), 24100085.
20 Singh S, Tripathi P, Kumar N, et al. Biosensors and Bioelectronics, 2016, 92, 280.
21 Luo L, Ou Y, Yang Y, et al. Journal of Hazardous Materials, 2022, 423, 127253.
22 Liu X L, Wang X H, Han Q S, et al. Talanta, 2019, 203, 227.
23 Borthakur P, Darabdhara G, Das M, et al. Sensors and Actuators B:Chemical, 2017, 244, 684.
24 Khan H, Yerramilli A S, D’oliveira A, et al. Canadian Journal of Chemical Engineering, 2020, 98(6), 1255.
25 Han S S, Wang Z J, Zhu W B, et al. Dalton Transactions, 2024, 53(10), 4737.
26 Salamon J, Simi A, Prabu H J, et al. Journal of Inorganic and Organometallic Polymers and Materials, 2024, 34(6), 2555.
27 Yao K L, Zhai M H, Ni Y H. Electrochimica Acta, 2019, 301, 87.
28 Sing K S W, Everett D H, Raw H. Pure and Applied Chemistry, 1985, 57(4), 603.
29 Ou H H, Yang P J, Lin L H, et al. Angewandte Chemie International Edition, 2017, 56(36), 10905.
30 Nabi G, Malik N, Raza W. Inorganic Chemistry Communications, 2020, 119, 108050.
31 Rakhi R B, Ahmed B, Hedhili M N, et al. Chemistry of Materials, 2015, 27(15), 5314.
32 Zhou X, Wang M, Su X. Sensors and Actuators B:Chemical, 2021, 338, 129777.
33 Gao Y Q, Zhao Y J, Xie H D, et al. Materials Reports, 2024, 38(12), 22110033(in Chinese).
高雅倩, 赵亚娟, 谢会东, 等. 材料导报, 2024, 38(12), 22110033.
34 Yang L, Wang D, Lv Y, et al. Carbon, 2019, 144, 8.
35 Lu W H, Chen J, Kong L S, et al. Sensors and Actuators B:Chemical, 2021, 333, 129560
36 Wu Y, Jiao L, Luo X, Xu W Q, et al. Small, 2019, 15(43), e1903108.
37 Wang T Y, Feng J X, Sun H, et al. Sensors and Actuators B:Chemical, 2023, 379, 133249.
38 Zhao F, Guo D Q, Tang X, et al. Talanta, 2024, 267, 125207.
39 Nong Y J, Zhang Y L, Hübner U, et al. Journal of Hazardous Materials, 2023, 446, 130660.
40 Hang L, Li X Q, Wang G L. Chinese Journal of Analytical Chemistry, 2020. 48(6), 703.
41 Fu F Y, Li L Y, Liu L J, et al. ACS Applied Materials and Interfaces, 2015, 7(4), 2597.
42 Aravindakumar C T, Oturan M, Aravind U. Environmental Science and Pollution Research, 2018, 25(21), 202811.