四面体DNA核酸适体生物传感器构建方法及应用

doi:10.11896/cldb.21050199

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Abstract The aptamer is a single-stranded DNA/RNA molecule, which has the advantages of easy and fast to synthesize, high stability, high specificity, wide range of target molecules and easy modification, etc. It has a good application prospect in biosensing. How to achieve the high activity and orderly immobilization of aptamers on the sensing interface is still a big problem for aptamer biosensors. Tetrahedral DNA is a three-dimensional DNA nanostructure composed of four single stranded DNA, which can improve the ability of aptamers at the sensing interface to recognize target molecules. Due to the specific A-T and G-C complementary pairing rules between DNA bases, the size of tetrahedral DNA three-dimensional nanostructures and the distance between adjacent DNA probe chains can be accurately controlled by adjusting the DNA strand length. Three dimensional nanostructures based on tetrahedral DNA are expected to solve the problem of high activity and orderly immobilization of aptamers at the sensing interface. In this paper, the structure and immobilization of tetrahedral DNA, the construction method and application of tetrahedral DNA aptamer biosensor are introduced. The problems faced by tetrahedral DNA aptamer biosensor are analyzed and its prospect is prospected, so as to provide reference for the construction of highly sensitive tetrahedral DNA aptamer biosensor.

Key words： tetrahedral DNA aptamer biosensor construction method

Published: Online: 2023-01-03

CLC number:	TP212.3
	TP212.9
	Q523+.5

Fund:Foundation of State Key Laboratory of NBC Protection for Civilian (SKLNBC2018-03).

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	Articles by authors
	LIU Zhiwei
	TONG Zhaoyang
	DU Bin
	WANG Jiang
	LIU Shuai

Cite this article:

LIU Zhiwei,TONG Zhaoyang,DU Bin, et al. Construction Method and Application of Aptamer Biosensor Based on Tetrahedral DNA[J]. Materials Reports, 2022, 36(24): 21050199-6.

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http://www.mater-rep.com/EN/10.11896/cldb.21050199 OR http://www.mater-rep.com/EN/Y2022/V36/I24/21050199

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