噬菌体生物传感器用于肠杆菌检测的研究进展

doi:10.11896/cldb.25050083

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Abstract Enterobacterial infections have become a global public health threat. Rapid and accurate detection of Enterobacteriaceae is critical for the effective prevention and control of these infections. However, current detection methods remain time-consuming, labor-intensive,and expensive, and lack the capacity for on-site application. Bacteriophages, owing to their environmental sustainability, host specificity, and capacity to discriminate viable bacteria, have emerged as promising candidates, providing a rapid, specific, and highly sensitive approach for detection of Enterobacteriaceae. In this review, we highlight the advantages of phage as recognition elements in biosensors, the immobilization strategies for phage-based sensors, and the applications of electrochemical, optical, and microfluidic phage-based sensors in Enterobacteriaceae detection, aiming to provide insights into the development of phage-based sensors.

Key words： bacteriophage biosensor Enterobacteriaceae electrochemical sensor optical sensor

Published: 25 June 2026 Online: 2026-07-08

CLC number:

R378

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	Articles by authors
	LIU Xue
	YAN Haiqin
	WANG Chunliang

Cite this article:

LIU Xue,YAN Haiqin,WANG Chunliang. Research Advances in Applying Phage-based Biosensors to Enterobacteriaceae Detection[J]. Materials Reports, 2026, 40(12): 25050083-6.

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https://www.mater-rep.com/EN/10.11896/cldb.25050083 OR https://www.mater-rep.com/EN/Y2026/V40/I12/25050083

1 Janda J M, Abbott S L. Clinical Microbiology Reviews, 2021, 34(2), e00174.
2 Bai Z Y, Wang W, Ji X F, et al. Journal of Biotechnology, 2021, 37(7), 2414 (in Chinese).
白芷烨, 汪雯, 吉小凤, 等. 生物工程学报, 2021, 37(7), 2414.
3 Yan K, Desai K, Gullapalli L, et al. Clinical Epidemiology, 2020, 12, 295.
4 Nazir J, Manzoor T, Saleem A, et al. Biomedicine Infectious Disease, 2025, 25(1), 84.
5 Nikiforov V V, Gao H, Zhou L, et al In:Advances in Experimental Medicine and Biology, Springer Netherlands, 2016, pp. 293.
6 Yang R, Atkinson S, Chen Z, et al. Zoonoses, 2023, 3(1), 5.
7 Cui Y, Yu C, Yan Y, et al. Proceedings of the National Academy of Sciences, 2013, 110(2), 577.
8 World Health Organization. Antimicrobial resistance, https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance, 2023.
9 World Health Organization. New report calls for urgent action to avert antimicrobial resistance crisis, 2019.
10 Wang X F. Argonaute-based fluorescent biosensor for the detection of salmonella typhimurium in food. Master’s Thesis, Huazhong Agricultural University, China, 2024 (in Chinese).
王绪丰. 基于Argonaute蛋白的荧光生物传感器检测食品中鼠伤寒沙门氏菌的研究. 硕士学位论文, 华中农业大学, 2024.
11 Ray M, Ray A, Singh S. Biosensors and Bioelectronics, 2017, 87(1), 708.
12 Ahovan Z A, Hashemi A, Plano L M D, et al. Nanomaterials, 2020, 10(3), 501.
13 Wang J, Zheng Y, Huang H, et al. Food Research International, 2024, 191, 114727.
14 Sun X C, Xu S Y, Li X Y, et al. Food and Fermentation Industries, 2023, 49(9), 340 (in Chinese).
孙新城, 许素月, 李侠颖, 等. 食品与发酵工业, 2023, 49(9), 340.
15 Bai J, Kim Y T, Ryu S, et al. Frontiers in Microbiology, 2016, 7, 474.
16 Wang J. Study on application of electrochemical biosensor based on phage SEP37 and its tail fiber protein in the decetion of Salmonella. Master’s Thesis, Huazhong Agricultural University, China, 2022 (in Chinese).
王吉. 基于噬菌体SEP37及其尾纤蛋白的电化学生物传感器在检测沙门氏菌中的应用研究. 硕士学位论文, 华中农业大学, 2022.
17 Sharma S, Chatterjee S, Datta S, et al. Folia Microbiologica, 2017, 62(1), 17.
18 Dou C, Xiong J, Gu Y, et al. Nature Microbiology, 2018, 3(11), 1285.
19 Hussain W, Ullah M W, Farooq U, et al. Biosensors and Bioelectronics, 2021, 177, 112973.
20 Li T, Li L P, Qu L. Biotechnology Bulletin, 2023, 39(6), 88 (in Chinese).
李托, 李陇平, 屈雷, 等. 生物技术通报, 2023, 39(6), 88.
21 Dion M B, Oechslin F, Moineau S. Nature Reviews Microbiology, 2020, 18(3), 125.
22 Wang D, Chen J, Nugen S R. Analytical Chemistry, 2017, 89(3), 1650.
23 Uddin K, Jalal M, Easwaran, et al. Annals of Microbiology, 2018, 68(10), 637.
24 Zhao X N. Study on the genome of Yersinia pestis phage Yep-phi and identification of its receptors. Ph. D. Thesis, Academy of Military Medical Sciences, Chinese People’s Liberation Army, China, 2013 (in Chinese).
赵向娜. 鼠疫耶尔森氏菌噬菌体Yep-phi基因组研究及其受体鉴定. 博士学位论文, 中国人民解放军军事医学科学院, 2013.
25 Yu Q, Liu P, Zhong Y H, et al. Chinese Journal of Control of Endemic Diseases, 2025, 40(1), 4 (in Chinese).
余琼, 刘攀, 钟佑宏, 等. 中国地方病防治, 2025, 40(1), 4.
26 Denyes J M, Dunne M, Steiner S, et al. Applied and environmental microbiology, 2017, 83(12), e00277.
27 Yang Q, Wang Y. Food Chemistry, 2025, 484, 144399.
28 Chen J, Alcaine S D, Jiang Z, et al. Analytical Chemistry, 2015, 87(17), 8977.
29 Hou P F, Lian J Q, Xiang D, et al. Food and Fermentation Technology, 2024, 60(3) 1 (in Chinese).
后鹏飞, 连军强, 向丹, 等. 食品与发酵科技, 2024, 60(3), 1.
30 Jiang S S, Zhao R H, Qiu C, et al. Chinese Journal of Veterinary Science, 2024, 44(11), 2400 (in Chinese).
姜姗杉, 赵日虹, 邱操, 等. 中国兽医学报, 2024, 44(11), 2400.
31 Li Y J. Mechanism and application of phage resistance transfer mediated by staphylococcus aureus temperate phage SapYZUs7. Master’s Thesis, Yangzhou University, China, 2024 (in Chinese).
李雅洁. 金黄色葡萄球菌温和噬菌体SapYZUs7介导的噬菌体抗性转移机制及其应用. 硕士学位论文, 扬州大学, 2024.
32 O’connell L, Marcoux P R, Roupioz Y. ACS Biomaterials Science & Engineering, 2021, 7(6), 1987.
33 Wang F, Horikawa S, Hu J, et al. Sensors, 2017, 17, 386.
34 Ktari T, Baccar H, Mejri M B, et al. International Journal of Electrochemistry, 2012, 2012, 421692.
35 Horikawa S, Bedi D, Li S, et al. Biosensors and Bioelectronics, 2011, 26(5), 2361.
36 Wang J, Li H, Li C, et al. Food Research International, 2022, 158, 111479.
37 Hyeon S H, Lim W K, Shin H J. Biotechnology and Applied Biochemistry, 2021, 68(1), 5.
38 Singh A, Glass N, Tolba M, et al. Biosensors and Bioelectronics, 2009, 24(12), 3645.
39 Edgar R, Mckinstry M, Hwang J, et al. Proceedings of the National Academy of Sciences, 2006, 103(13), 4841.
40 Lee S M, Lee S, Lee S H, et al. Sensors and Actuators B:Chemical, 2023, 393, 134244.
41 Quintela I A, Wu V C H. RSC Advances, 2020, 10(59), 35765.
42 Bhardwaj N, Bhardwaj S K, Mehta J, et al. Analytical Biochemistry, 2016, 505, 18.
43 Zhou Y, Marar A, Kner P, et al. Analytical Chemistry, 2017, 89(11), 5734.
44 Farooq U, Ullah M W, Yang Q, et al. Biosensors and Bioelectronics, 2020, 157, 112163.
45 Hussain W, Wang H, Yang X, et al. Biosensors, 2024, 14, 500.
46 Zhu S, Liu T, Tao Z, et al. Sensors and Actuators B, 2025, 423, 136729.
47 Sedki M, Chen X, Chen C, et al. Biosensors and Bioelectronics, 2020, 148, 111794.
48 Zhou Y, Li Z, Huang J, et al. Bioelectrochemistry, 2023, 150, 108345.
49 Abdelhamied N, Abdelrahman F, El-shibiny A, et al. Scientific Reports, 2023, 13(1), 3498.
50 Yang Q, Deng S, Xu J, et al. Microchimica Acta, 2021, 188(4), 107.
51 Ding Y, Zhang Y, Huang C, et al. Talanta, 2024, 270, 125561.
52 Xu Z H. Detection of Escherichia coli based on phage recognition and AIE fluorescent labeling. Master ’s Thesis, Huazhong Agricultural University, China, 2023 (in Chinese).
徐祖辉. 基于噬菌体识别和AIE荧光标记检测大肠杆菌. 硕士学位论文, 华中农业大学, 2023.
53 Zeng Q, Deng T, Yang Y, et al. Journal of Hazardous Materials, 2025, 492, 138295.
54 Arya S K, Singh A, Naidoo R, et al. The Analyst, 2011, 136(3), 486.
55 Feng N, Li Y, Zhao Y, et al. ACS Sensors, 2025, 10(2), 785.
56 Zhang Z W. Research on instant detection technology of pathogenic microorganisms based on electrochemical sensors. Ph. D. Thesis, Academy of Military Sciences, China, 2024 (in Chinese).
张志伟. 基于电化学传感器的病原微生物即时检测技术研究. 博士学位论文, 军事科学院, 2024.