噬菌体生物传感器用于肠杆菌检测的研究进展

doi:10.11896/cldb.25050083

材料导报

2026, Vol. 40

Issue (12): 25050083-6 https://doi.org/10.11896/cldb.25050083

高分子与聚合物基复合材料

噬菌体生物传感器用于肠杆菌检测的研究进展

刘雪¹, 严海芹^1,*, 王春亮²

1 蚌埠医科大学基础医学院感染与免疫安徽省重点实验室,安徽蚌埠 233000
2 中国医学科学院北京协和医学院病原生物学研究所,北京 100730

Research Advances in Applying Phage-based Biosensors to Enterobacteriaceae Detection

LIU Xue¹, YAN Haiqin^1,*, WANG Chunliang²

1 Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu Medical University, Bengbu 233000, Anhui, China
2 Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China

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摘要肠杆菌感染每年导致大量患者死亡,开发快速精准的检测技术已成为防治肠杆菌病原体引发的感染性疾病的关键手段。然而,目前肠杆菌检测仍主要依赖常规细菌检测法,存在耗时、费力且操作难度大等缺点。噬菌体具备结构简单、宿主特异性强、易培养、可区分细菌存活状态等特性,成为开发生物传感器的理想材料。基于噬菌体的肠杆菌检测传感器操作便捷,特异性强,灵敏性高,在临床诊断和食品安全监测等领域展现出重要的应用价值。本文诠释了噬菌体作为传感器识别元件的优势,阐明了噬菌体传感器的固定化策略,探讨了电化学、光学及微流控传感技术在噬菌体传感器检测肠杆菌中的应用和进展,可为噬菌体传感器发展和肠杆菌检测提供参考。

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关键词: 噬菌体生物传感器肠杆菌电化学传感器光学传感器

Abstract: Enterobacterial infections have become a global public health threat. Rapid and accurate detection of Enterobacteriaceae is critical for the effective prevention and control of these infections. However, current detection methods remain time-consuming, labor-intensive,and expensive, and lack the capacity for on-site application. Bacteriophages, owing to their environmental sustainability, host specificity, and capacity to discriminate viable bacteria, have emerged as promising candidates, providing a rapid, specific, and highly sensitive approach for detection of Enterobacteriaceae. In this review, we highlight the advantages of phage as recognition elements in biosensors, the immobilization strategies for phage-based sensors, and the applications of electrochemical, optical, and microfluidic phage-based sensors in Enterobacteriaceae detection, aiming to provide insights into the development of phage-based sensors.

Key words: bacteriophage biosensor Enterobacteriaceae electrochemical sensor optical sensor

出版日期: 2026-06-25 发布日期: 2026-07-08

ZTFLH:

R378

基金资助: 国家自然科学基金(31800120)

通讯作者: ^*严海芹,博士,蚌埠医科大学基础医学院副教授、硕士研究生导师,主要研究病原菌的环境适应性及其机制等。yanhq@bbmu.edu.cn

作者简介: 刘雪,蚌埠医科大学基础医学院硕士研究生,导师为严海芹,主要研究病原菌的环境适应性及其机制。

引用本文:

刘雪, 严海芹, 王春亮. 噬菌体生物传感器用于肠杆菌检测的研究进展[J]. 材料导报, 2026, 40(12): 25050083-6.
LIU Xue, YAN Haiqin, WANG Chunliang. Research Advances in Applying Phage-based Biosensors to Enterobacteriaceae Detection. Materials Reports, 2026, 40(12): 25050083-6.

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https://www.mater-rep.com/CN/10.11896/cldb.25050083 或 https://www.mater-rep.com/CN/Y2026/V40/I12/25050083

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