羊毛角蛋白中巯基含量的测定及其应用

doi:10.11896/cldb.20040075

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Abstract Wool keratin contains a large number of cysteine residues. The interaction between the sulfhydryl groups in these cysteine residues is important for maintaining the secondary and tertiary structure of the protein. In order to determine the sulfhydryl group content in wool keratin, the responsiveness of the sulfhydryl group by the 4,4'-dipyridyldisulfide (DPS) method and the classic Ellman's reagent method was compared in detail in different denaturants and pH systems. The study found that the DPS method had higher sulfhydryl responsiveness than the Ellman's reagent method at pH 7.0, 8 mol/L urea, 1% sodium dodecyl sulfate (SDS) and 3 mmol/L ethylenediamine tetraacetic acid (EDTA) buffer system. Therefore, the oxidation of sulfhydryl groups in wool keratin by hydrogen peroxide solution and the destruction of disulfide bonds in wool fiber protein by high temperature were verified by the DPS method in the above buffer system. The experimental results showed that the DPS method could be accurately and reliably applied to the determination of sulfhydryl content in wool keratin.

Key words： wool keratin sulfhydryl group 4,4'-dipyridyl disulfide (DPS) method Ellman's reagent method

Published: 03 August 2021

CLC number:

O629.73

Fund:National Natural Science Foundation of China (31771039), the National Key R&D Program of China (2017YFB0309200), Fundamental Research Funds for the Central Universities (JUSRP51717A).

About author:: Guang Chen is currently a master degree candidate in School of Textile and Apparel of Jiangnan University. She is focusing on dissolution and regeneration of wool keratin and preparation of its composites materials.
Jiugang Yuan received his Ph.D. degree from Jiangnan University in 2010. He is currently an associate professor of Key laboratory of Eco-textiles, Ministry of Education, Jiangnan University, mainly engaged in the research of natural fibers, including their extraction and functional modification.

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	CHEN Guang
	YUAN Jiugang
	XIANG Yu
	FAN Xuerong
	WANG Ping
	WANG Qiang

Cite this article:

CHEN Guang,YUAN Jiugang,XIANG Yu, et al. Determination of Sulfhydryl Content in Wool Keratin and Its Application[J]. Materials Reports, 2021, 35(14): 14176-14179.

URL:

http://www.mater-rep.com/EN/10.11896/cldb.20040075 OR http://www.mater-rep.com/EN/Y2021/V35/I14/14176

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