基于探针标记的免疫层析试纸及其应用

doi:10.11896/cldb.19060033

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Abstract Immunochromatographic test strips (ICTSs) are a new type oftest strips based on immunochromatographic detection technology.Because of the combination of the high specificity of immunotechnology with the superior separation ability of chromatography, ICTSs have many advantages, such as portability, less time-consuming, relatively stable test results, low price and so on. At present, ICTSs are widely used in the detection of various biological molecules, chemical pollutants and infection factors.
In recent years, the development of ICTSs has been greatly limited due to its low sensitivity, detection limit and specificity.The factor that primarily affects the performance of the test strips is the label used as a probe.In order to improve the performance of the test strips, researchers mainly explored two aspects: (ⅰ) signal amplification by adding enhancers to the test strips; (ⅱ) in addition to the commonly used colloidal gold, quantum dots and latex particles, new labels such as upconverting fluorescent particles, carbon nanoparticles, liposomes, magnetic nano-particles, and selenium nanoparticles to improve test strips performance were studied.
At present, fruitful results have been achieved through these studies.Colloidal gold is one of the most commonly used probes in ICTSs. Although it has a wide detection range, convenient operation, speed and strong specificity, its sensitivity is low.In recent years, the detection limit and sensitivity have been significantly improved by adding enhancers (such as HAuCl₄ and NH₂OH·HCl) to colloidal gold ICTSs.Compared with colloidal gold or colour latex particles, the sensitivity of upconverting fluorescent particles for ICTSs can also be increased by 10—100 times under the same biological conditions.In addition, selenium nanoparticles have good biocompatibility and low cost, so the research and development of selenium nanoparticles as ICTSs immunoprobe has broad prospects.
This review describes the basic structure of ICTSs and two detection methods: competitive format and sandwich format,and the different designs of ICTSs in recent years (adding multiple test lines to the same test strips, different shape test strips and lateral flow microarray).Based on the influence of different probe labels on ICTSs, several widely studied labels have been introduced in recent years, the advantages and disadvantages of different labels and their application status are analyzed. Finally, we review the applications of ICTSs in human medical diseases, agricultural production and food safety. In the future, the emphasis should be put on exploring composite probes in order to meet the goal of “fast, convenient, specific and sensitive” in the field of detection.

Key words： immunochromatographic test strips nitrocellulose membrane label probe

Published: 14 July 2020

CLC number:

O652

Fund:This work was financially supported by the National Natural Science Foundation of China (51801001), the Provincial Key Research and Development Program of Shaanxi (2019GY-197), the Basic Research Plan of Natural Science of Shaanxi (2015JM5215), China Post-Doctoral Science Foundation Project (2016M601878), Baoji Science and Technology Plan Project (16RKX1-29), and Shaanxi Thousand People Program Youth Project.

About author:: Jinzi Ding received her B.E. degree in Baoji University of Arts and Sciences in 2018. She is currently pursuing her master degree in optical engineering of Baoji University of Arts and Sciences under the supervision of teac-her. Ailing Feng. Her research has focused on biosensor.
Ailing Feng received her B.E. degree in applied che-mistry from Chang'an University, M.S. degree in pharmaceutical chemistry from the Fourth Military Medical University and received her Ph.D. degree in materials science and engineering from Xi'an Jiao Tong University in 2002, 2005 and 2011, respectively. After postdocto-ral research at Xi'an Jiao Tong University, she is currently a professor and master supervisor in Baoji University of Arts & Sciences. She is also a winner in the first batch of “Shaanxi young thousand talents plan” and the first batch of “Hengqu scholars” at Baoji University of Arts & Sciences. During 2016—2018, as a visiting scholar, she did some research in Washington University in St. Louis, USA. Her research directions include: preparation of magnesium matrix composites and their application in bone tissue implantation, growth mechanism of nano hydroxyapatite fibers, study on the controllable synthesis and fluorescence enhancement mechanism of rare earth upcversion materials, and design and research of new insulation micro-nano composites with high thermal conductive and high dielectric properties. As a first author or corres-
pondent author, she has published more than 20 papers in domestic and fo-reign acade-mic journals. Total number of citations reached 970 (data from Google scho-lar). Eight articles were selected as highly cited papers in the ESI database. And two of them were selected hot papers in the ESI database as well. She applied for 8 national invention patents, of which 4 were autho-rized. She presided over 1 national natural science foundation of China, hosted 1 theprovincial key research and development program of Shaanxi, and presided over 8 other provincial level and municipal research projects, and participated in the national major instrument development project as a key member, and major international (regional) cooperative research project of the natural science fund committee. She is also the first prize winner of outstanding academic papers in the natural sciences of Shaanxi province.

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Cite this article:

DING Jinzi,FENG Ailing,XU Rong, et al. Immunochromatographic Test Strips Based on Probe Labels and Their Application[J]. Materials Reports, 2020, 34(15): 15174-15183.

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http://www.mater-rep.com/EN/10.11896/cldb.19060033 OR http://www.mater-rep.com/EN/Y2020/V34/I15/15174

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